scvelo

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RNA velocity analysis with scVelo. Estimate cell state transitions from unspliced/spliced mRNA dynamics, infer trajectory directions, compute latent time, and identify driver genes in single-cell RNA-seq data. Complements Scanpy/scVI-tools for trajectory inference.

AI & Automation 26,817 stars 2774 forks Updated today MIT

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Skill Content

# scVelo — RNA Velocity Analysis ## Overview scVelo is the leading Python package for RNA velocity analysis in single-cell RNA-seq data. It infers cell state transitions by modeling the kinetics of mRNA splicing — using the ratio of unspliced (pre-mRNA) to spliced (mature mRNA) abundances to determine whether a gene is being upregulated or downregulated in each cell. This allows reconstruction of developmental trajectories and identification of cell fate decisions without requiring time-course data. **Installation:** `pip install scvelo` **Key resources:** - Documentation: https://scvelo.readthedocs.io/ - GitHub: https://github.com/theislab/scvelo - Paper: Bergen et al. (2020) Nature Biotechnology. PMID: 32747759 ## When to Use This Skill Use scVelo when: - **Trajectory inference from snapshot data**: Determine which direction cells are differentiating - **Cell fate prediction**: Identify progenitor cells and their downstream fates - **Driver gene identification**: Find genes whose dynamics best explain observed trajectories - **Developmental biology**: Model hematopoiesis, neurogenesis, epithelial-to-mesenchymal transitions - **Latent time estimation**: Order cells along a pseudotime derived from splicing dynamics - **Complement to Scanpy**: Add directional information to UMAP embeddings ## Prerequisites scVelo requires count matrices for both **unspliced** and **spliced** RNA. These are generated by: 1. **STARsolo** or **kallisto|bustools** with `lamanno` mode 2. *...

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Author
K-Dense-AI
Repository
K-Dense-AI/scientific-agent-skills
Created
7 months ago
Last Updated
today
Language
Python
License
MIT

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